Author: kikogarcia

Within the domain of high-throughput RNA sequencing, the precise quantification of gene expression levels and the detection of infrequent variants play a pivotal role in illuminating the intricacies of biological phenomena and disease mechanisms. Nevertheless, several technical obstacles, including PCR duplicates and sequencing errors, impede the fidelity and exactness of RNA-seq data. The advent of…

Introduction to N1-methyladenosine (m1A) Sequencing In eukaryotic cells, N1-methyladenosine (m1A) is found in thousands of different gene transcripts (from yeast to mammals). The majority of m1A methylation modifications occur in the 5’UTR region of mRNA, and it is a common post-transcriptional modification in tRNA and rRNA in eukaryotes. m1A at the first and second nucleotides…